Relative Analysis of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Focusing on Nucleic Acid Extraction.
(LNJNbio Polystyrene Microspheres)
In the field of modern-day biotechnology, microsphere materials are extensively made use of in the extraction and purification of DNA and RNA as a result of their high specific surface, good chemical security and functionalized surface area properties. Amongst them, polystyrene (PS) microspheres and their obtained polystyrene carboxyl (CPS) microspheres are among both most extensively studied and used products. This post is provided with technical support and data analysis by Shanghai Lingjun Biotechnology Co., Ltd., intending to methodically compare the efficiency distinctions of these two types of products in the procedure of nucleic acid removal, covering essential indicators such as their physicochemical residential or commercial properties, surface adjustment capacity, binding performance and healing rate, and show their relevant scenarios through experimental information.
Polystyrene microspheres are uniform polymer fragments polymerized from styrene monomers with good thermal security and mechanical stamina. Its surface is a non-polar framework and typically does not have energetic useful groups. Consequently, when it is directly used for nucleic acid binding, it requires to depend on electrostatic adsorption or hydrophobic action for molecular fixation. Polystyrene carboxyl microspheres present carboxyl functional teams (– COOH) on the basis of PS microspheres, making their surface efficient in more chemical coupling. These carboxyl teams can be covalently bonded to nucleic acid probes, healthy proteins or other ligands with amino groups via activation systems such as EDC/NHS, thereby attaining more secure molecular fixation. Therefore, from an architectural point of view, CPS microspheres have more advantages in functionalization capacity.
Nucleic acid extraction generally consists of steps such as cell lysis, nucleic acid release, nucleic acid binding to solid stage carriers, cleaning to eliminate contaminations and eluting target nucleic acids. In this system, microspheres play a core function as solid stage carriers. PS microspheres mainly depend on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding effectiveness has to do with 60 ~ 70%, however the elution efficiency is low, only 40 ~ 50%. In contrast, CPS microspheres can not only utilize electrostatic effects yet additionally accomplish more solid fixation through covalent bonding, reducing the loss of nucleic acids throughout the washing procedure. Its binding performance can get to 85 ~ 95%, and the elution effectiveness is also boosted to 70 ~ 80%. Furthermore, CPS microspheres are likewise significantly much better than PS microspheres in regards to anti-interference ability and reusability.
In order to validate the performance differences in between both microspheres in real operation, Shanghai Lingjun Biotechnology Co., Ltd. conducted RNA removal experiments. The speculative samples were originated from HEK293 cells. After pretreatment with typical Tris-HCl barrier and proteinase K, 5 mg/mL PS and CPS microspheres were made use of for removal. The results revealed that the ordinary RNA return removed by PS microspheres was 85 ng/ μL, the A260/A280 proportion was 1.82, and the RIN value was 7.2, while the RNA yield of CPS microspheres was raised to 132 ng/ μL, the A260/A280 ratio was close to the excellent worth of 1.91, and the RIN value got to 8.1. Although the operation time of CPS microspheres is a little longer (28 mins vs. 25 mins) and the price is greater (28 yuan vs. 18 yuan/time), its extraction high quality is significantly boosted, and it is preferable for high-sensitivity discovery, such as qPCR and RNA-seq.
( SEM of LNJNbio Polystyrene Microspheres)
From the perspective of application scenarios, PS microspheres are suitable for massive screening jobs and preliminary enrichment with reduced demands for binding uniqueness as a result of their inexpensive and easy procedure. However, their nucleic acid binding ability is weak and quickly influenced by salt ion focus, making them unsuitable for long-lasting storage or repeated usage. In contrast, CPS microspheres are suitable for trace example removal because of their abundant surface area practical groups, which help with further functionalization and can be used to construct magnetic bead discovery kits and automated nucleic acid removal systems. Although its prep work process is reasonably complicated and the expense is reasonably high, it reveals stronger adaptability in scientific research and professional applications with strict requirements on nucleic acid removal efficiency and purity.
With the rapid advancement of molecular medical diagnosis, genetics editing and enhancing, fluid biopsy and various other fields, greater needs are put on the effectiveness, purity and automation of nucleic acid removal. Polystyrene carboxyl microspheres are progressively changing standard PS microspheres because of their superb binding efficiency and functionalizable attributes, becoming the core choice of a brand-new generation of nucleic acid extraction products. Shanghai Lingjun Biotechnology Co., Ltd. is likewise constantly enhancing the particle size distribution, surface density and functionalization efficiency of CPS microspheres and creating matching magnetic composite microsphere items to satisfy the requirements of scientific diagnosis, clinical research establishments and industrial clients for top quality nucleic acid removal services.
Supplier
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need dna isolation and extraction, please feel free to contact us at sales01@lingjunbio.com.
All articles and pictures are from the Internet. If there are any copyright issues, please contact us in time to delete.
Inquiry us
